Part:BBa_K2121008:Design

Melibiose Inducible Promoter

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 142
  Illegal SpeI site found at 110
  Illegal PstI site found at 39
  Illegal PstI site found at 274
  Illegal PstI site found at 436
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 142
  Illegal NheI site found at 4972
  Illegal SpeI site found at 110
  Illegal PstI site found at 39
  Illegal PstI site found at 274
  Illegal PstI site found at 436
  Illegal NotI site found at 2846
  Illegal NotI site found at 4379
  Illegal NotI site found at 5669
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 142
  Illegal BamHI site found at 1554
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 142
  Illegal SpeI site found at 110
  Illegal PstI site found at 39
  Illegal PstI site found at 274
  Illegal PstI site found at 436
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 142
  Illegal SpeI site found at 110
  Illegal PstI site found at 39
  Illegal PstI site found at 274
  Illegal PstI site found at 436
  Illegal NgoMIV site found at 253
  Illegal NgoMIV site found at 682
  Illegal NgoMIV site found at 2291
  Illegal NgoMIV site found at 2465
  Illegal NgoMIV site found at 2515
  Illegal NgoMIV site found at 2519
  Illegal NgoMIV site found at 2546
  Illegal AgeI site found at 92
  Illegal AgeI site found at 5466
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1521
  Illegal BsaI.rc site found at 793
  Illegal SapI site found at 708
  Illegal SapI site found at 6060
  Illegal SapI.rc site found at 5597
  

Design Notes

Utilize native suppression mechanism in S. meliloti.

Construct design

Source

Mostafavi, Mina, et al. "Analysis of a taurine-dependent promoter in Sinorhizobium meliloti that offers tight modulation of gene expression." BMC microbiology 14.1 (2014): 1.